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dual index sequencing adapters nebnext multiplex oligos for illumina kit neb ultra ii master mix  (New England Biolabs)


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    New England Biolabs dual index sequencing adapters nebnext multiplex oligos for illumina kit neb ultra ii master mix
    Dual Index Sequencing Adapters Nebnext Multiplex Oligos For Illumina Kit Neb Ultra Ii Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 3150 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/neb+e6440s/NEBNext+Multiplex+Oligos+for+Illumina/pmc13649568-393-14-17
    Average 97 stars, based on 3150 article reviews
    dual index sequencing adapters nebnext multiplex oligos for illumina kit neb ultra ii master mix - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Multiplex Assay:

    Article Title: Genome binding properties of Zic transcription factors underlie their changing functions during neuronal maturation
    Article Snippet: .. CUT&RUN libraries were made using the NEB Ultra II DNA Library Prep Kit for Illumina (NEB E7645L), and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (NEB E6440S). .. Library cleanup was performed prior to and after PCR amplification using 0.8X Kapa Hyperpure beads (Roche 08963851001).

    Article Title: Genome binding properties of Zic transcription factors underlie their changing functions during neuronal maturation
    Article Snippet: .. CUT&RUN libraries were made using the NEB Ultra II DNA Library Prep Kit for Illumina (NEB E7645L) and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (NEB E6440S). .. Library cleanup was performed prior to and after PCR amplification using 0.8X Kapa Hyperpure beads (Roche 08963851001).

    Article Title: RLF/ZFP292 stabilize CoREST-linked LSD1 engagement at bivalent promoters to safeguard pluripotency.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays In-Fusion HD Cloning Kit Takara 639650 DNA Ligation Kit ver. .. 2.1 Takara 6022 Lipofectamine 3000 Thermo Fisher Scientific L3000008 Leukocyte Alkaline Phosphatase Kit Sigma-Aldrich 86R ReverTra Ace qPCR RT Master Mix TOYOBO FSQ-201 TB Green Premix Ex Taq II Takara RR820A SYBR Green qPCR Master Mix Thermo Fisher Scientific A66732 QIAquick PCR Purification Kit Qiagen 28104 NEBNext Ultra II DNA Library Prep Kit for Illumina NEB E7645L NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) NEB E7600S NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 2) NEB E7780S NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) NEB E6440S Deposited data Raw and analyzed data (crosslinked IP-MS) This paper PXD075871 Raw and analyzed data (native IP-MS) This paper PXD075910 Raw and analyzed data (ChIP-seq) This paper GSE297949 Raw and analyzed data (RNA-seq) This paper GSE297950 REST ChIP-seq in mouse ESCs Arnold et al.37 GSE27148 ENCODE Blacklist for mm10 genome Amemiya et al.55 https://github.com/Boyle-Lab/Blacklist/ blob/master/lists/mm10-blacklist.v2.bed.gz ChromHMM_mESC_mm10 Pintacuda et al.36 https://github.com/guifengwei/ ChromHMM_mESC_mm10/blob/master/ mESC_E14_12_segments.bed.gz Original western blot images and fluorescence microscopy images This paper Mendeley data https://doi.org/ 10.17632/2j3895t9rh.1 Experimental models: Cell lines TT2 mESCs WT Gifted from Dr. Tachibana RRID: CVCL_C318 TT2 mESCs Rlf KO This paper N/A TT2 mESCs Zfp292 KO This paper N/A TT2 mESCs Rlf/Zfp292 DKO This paper N/A TT2 mESCs Rex1-EGFP This paper N/A TT2 mESCs Rlf/Zfp292 DKO Rex1-EGFP This paper N/A TT2 mESCs Zfp292 KO + Rlf-dTAG-2HA This paper N/A TT2 mESCs Zfp292/Zfp654 DKO + Rlf-dTAG-2HA This paper N/A TT2 mESCs Rlf-FLAG This paper N/A TT2 mESCs Zfp292-FLAG This paper N/A TT2 mESCs Zfp654-FLAG This paper N/A TT2 mESCs Rcor1-FLAG This paper N/A TT2 mESCs Rcor2-FLAG This paper N/A TT2 mESCs Rlf/Zfp292 DKO Rcor2-FLAG This paper N/A TT2 mESCs Rest-dTAG-FLAG This paper N/A Oligonucleotides See Table S2 for all oligonucleotides This paper N/A (Continued on next page) 16 Cell Reports 45, 117293, April 28, 2026 .. TT2 mESCs are kindly gifted from Dr. Tachibana in Osaka university. mESCs were cultured on dishes coated with 0.1% gelatin in DMEM (Nacalai tesque, 08458-16) supplemented with 10% Knockout Serum Replacement (Gibco, 10828028), 1% FBS (HyClone, SH30070.03), 1× sodium pyruvate (Wako, 190–14881), 1× non-essential amino acids (Wako, 139–15651), 0.1 mM 2-mercaptoethanol (Wako, 133–14571), 1× penicillin-streptomycin (Gibco, 15140122), and LIF (produced in-house) at 37◦C with 5% CO2. mESCs were passaged every 2–3 days using 0.05% trypsin-EDTA (Nacalai tesque, 32777-44).

    Real-time Polymerase Chain Reaction:

    Article Title: RLF/ZFP292 stabilize CoREST-linked LSD1 engagement at bivalent promoters to safeguard pluripotency.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays In-Fusion HD Cloning Kit Takara 639650 DNA Ligation Kit ver. .. 2.1 Takara 6022 Lipofectamine 3000 Thermo Fisher Scientific L3000008 Leukocyte Alkaline Phosphatase Kit Sigma-Aldrich 86R ReverTra Ace qPCR RT Master Mix TOYOBO FSQ-201 TB Green Premix Ex Taq II Takara RR820A SYBR Green qPCR Master Mix Thermo Fisher Scientific A66732 QIAquick PCR Purification Kit Qiagen 28104 NEBNext Ultra II DNA Library Prep Kit for Illumina NEB E7645L NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) NEB E7600S NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 2) NEB E7780S NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) NEB E6440S Deposited data Raw and analyzed data (crosslinked IP-MS) This paper PXD075871 Raw and analyzed data (native IP-MS) This paper PXD075910 Raw and analyzed data (ChIP-seq) This paper GSE297949 Raw and analyzed data (RNA-seq) This paper GSE297950 REST ChIP-seq in mouse ESCs Arnold et al.37 GSE27148 ENCODE Blacklist for mm10 genome Amemiya et al.55 https://github.com/Boyle-Lab/Blacklist/ blob/master/lists/mm10-blacklist.v2.bed.gz ChromHMM_mESC_mm10 Pintacuda et al.36 https://github.com/guifengwei/ ChromHMM_mESC_mm10/blob/master/ mESC_E14_12_segments.bed.gz Original western blot images and fluorescence microscopy images This paper Mendeley data https://doi.org/ 10.17632/2j3895t9rh.1 Experimental models: Cell lines TT2 mESCs WT Gifted from Dr. Tachibana RRID: CVCL_C318 TT2 mESCs Rlf KO This paper N/A TT2 mESCs Zfp292 KO This paper N/A TT2 mESCs Rlf/Zfp292 DKO This paper N/A TT2 mESCs Rex1-EGFP This paper N/A TT2 mESCs Rlf/Zfp292 DKO Rex1-EGFP This paper N/A TT2 mESCs Zfp292 KO + Rlf-dTAG-2HA This paper N/A TT2 mESCs Zfp292/Zfp654 DKO + Rlf-dTAG-2HA This paper N/A TT2 mESCs Rlf-FLAG This paper N/A TT2 mESCs Zfp292-FLAG This paper N/A TT2 mESCs Zfp654-FLAG This paper N/A TT2 mESCs Rcor1-FLAG This paper N/A TT2 mESCs Rcor2-FLAG This paper N/A TT2 mESCs Rlf/Zfp292 DKO Rcor2-FLAG This paper N/A TT2 mESCs Rest-dTAG-FLAG This paper N/A Oligonucleotides See Table S2 for all oligonucleotides This paper N/A (Continued on next page) 16 Cell Reports 45, 117293, April 28, 2026 .. TT2 mESCs are kindly gifted from Dr. Tachibana in Osaka university. mESCs were cultured on dishes coated with 0.1% gelatin in DMEM (Nacalai tesque, 08458-16) supplemented with 10% Knockout Serum Replacement (Gibco, 10828028), 1% FBS (HyClone, SH30070.03), 1× sodium pyruvate (Wako, 190–14881), 1× non-essential amino acids (Wako, 139–15651), 0.1 mM 2-mercaptoethanol (Wako, 133–14571), 1× penicillin-streptomycin (Gibco, 15140122), and LIF (produced in-house) at 37◦C with 5% CO2. mESCs were passaged every 2–3 days using 0.05% trypsin-EDTA (Nacalai tesque, 32777-44).

    SYBR Green Assay:

    Article Title: RLF/ZFP292 stabilize CoREST-linked LSD1 engagement at bivalent promoters to safeguard pluripotency.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays In-Fusion HD Cloning Kit Takara 639650 DNA Ligation Kit ver. .. 2.1 Takara 6022 Lipofectamine 3000 Thermo Fisher Scientific L3000008 Leukocyte Alkaline Phosphatase Kit Sigma-Aldrich 86R ReverTra Ace qPCR RT Master Mix TOYOBO FSQ-201 TB Green Premix Ex Taq II Takara RR820A SYBR Green qPCR Master Mix Thermo Fisher Scientific A66732 QIAquick PCR Purification Kit Qiagen 28104 NEBNext Ultra II DNA Library Prep Kit for Illumina NEB E7645L NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) NEB E7600S NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 2) NEB E7780S NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) NEB E6440S Deposited data Raw and analyzed data (crosslinked IP-MS) This paper PXD075871 Raw and analyzed data (native IP-MS) This paper PXD075910 Raw and analyzed data (ChIP-seq) This paper GSE297949 Raw and analyzed data (RNA-seq) This paper GSE297950 REST ChIP-seq in mouse ESCs Arnold et al.37 GSE27148 ENCODE Blacklist for mm10 genome Amemiya et al.55 https://github.com/Boyle-Lab/Blacklist/ blob/master/lists/mm10-blacklist.v2.bed.gz ChromHMM_mESC_mm10 Pintacuda et al.36 https://github.com/guifengwei/ ChromHMM_mESC_mm10/blob/master/ mESC_E14_12_segments.bed.gz Original western blot images and fluorescence microscopy images This paper Mendeley data https://doi.org/ 10.17632/2j3895t9rh.1 Experimental models: Cell lines TT2 mESCs WT Gifted from Dr. Tachibana RRID: CVCL_C318 TT2 mESCs Rlf KO This paper N/A TT2 mESCs Zfp292 KO This paper N/A TT2 mESCs Rlf/Zfp292 DKO This paper N/A TT2 mESCs Rex1-EGFP This paper N/A TT2 mESCs Rlf/Zfp292 DKO Rex1-EGFP This paper N/A TT2 mESCs Zfp292 KO + Rlf-dTAG-2HA This paper N/A TT2 mESCs Zfp292/Zfp654 DKO + Rlf-dTAG-2HA This paper N/A TT2 mESCs Rlf-FLAG This paper N/A TT2 mESCs Zfp292-FLAG This paper N/A TT2 mESCs Zfp654-FLAG This paper N/A TT2 mESCs Rcor1-FLAG This paper N/A TT2 mESCs Rcor2-FLAG This paper N/A TT2 mESCs Rlf/Zfp292 DKO Rcor2-FLAG This paper N/A TT2 mESCs Rest-dTAG-FLAG This paper N/A Oligonucleotides See Table S2 for all oligonucleotides This paper N/A (Continued on next page) 16 Cell Reports 45, 117293, April 28, 2026 .. TT2 mESCs are kindly gifted from Dr. Tachibana in Osaka university. mESCs were cultured on dishes coated with 0.1% gelatin in DMEM (Nacalai tesque, 08458-16) supplemented with 10% Knockout Serum Replacement (Gibco, 10828028), 1% FBS (HyClone, SH30070.03), 1× sodium pyruvate (Wako, 190–14881), 1× non-essential amino acids (Wako, 139–15651), 0.1 mM 2-mercaptoethanol (Wako, 133–14571), 1× penicillin-streptomycin (Gibco, 15140122), and LIF (produced in-house) at 37◦C with 5% CO2. mESCs were passaged every 2–3 days using 0.05% trypsin-EDTA (Nacalai tesque, 32777-44).

    Polymerase Chain Reaction:

    Article Title: RLF/ZFP292 stabilize CoREST-linked LSD1 engagement at bivalent promoters to safeguard pluripotency.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays In-Fusion HD Cloning Kit Takara 639650 DNA Ligation Kit ver. .. 2.1 Takara 6022 Lipofectamine 3000 Thermo Fisher Scientific L3000008 Leukocyte Alkaline Phosphatase Kit Sigma-Aldrich 86R ReverTra Ace qPCR RT Master Mix TOYOBO FSQ-201 TB Green Premix Ex Taq II Takara RR820A SYBR Green qPCR Master Mix Thermo Fisher Scientific A66732 QIAquick PCR Purification Kit Qiagen 28104 NEBNext Ultra II DNA Library Prep Kit for Illumina NEB E7645L NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) NEB E7600S NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 2) NEB E7780S NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) NEB E6440S Deposited data Raw and analyzed data (crosslinked IP-MS) This paper PXD075871 Raw and analyzed data (native IP-MS) This paper PXD075910 Raw and analyzed data (ChIP-seq) This paper GSE297949 Raw and analyzed data (RNA-seq) This paper GSE297950 REST ChIP-seq in mouse ESCs Arnold et al.37 GSE27148 ENCODE Blacklist for mm10 genome Amemiya et al.55 https://github.com/Boyle-Lab/Blacklist/ blob/master/lists/mm10-blacklist.v2.bed.gz ChromHMM_mESC_mm10 Pintacuda et al.36 https://github.com/guifengwei/ ChromHMM_mESC_mm10/blob/master/ mESC_E14_12_segments.bed.gz Original western blot images and fluorescence microscopy images This paper Mendeley data https://doi.org/ 10.17632/2j3895t9rh.1 Experimental models: Cell lines TT2 mESCs WT Gifted from Dr. Tachibana RRID: CVCL_C318 TT2 mESCs Rlf KO This paper N/A TT2 mESCs Zfp292 KO This paper N/A TT2 mESCs Rlf/Zfp292 DKO This paper N/A TT2 mESCs Rex1-EGFP This paper N/A TT2 mESCs Rlf/Zfp292 DKO Rex1-EGFP This paper N/A TT2 mESCs Zfp292 KO + Rlf-dTAG-2HA This paper N/A TT2 mESCs Zfp292/Zfp654 DKO + Rlf-dTAG-2HA This paper N/A TT2 mESCs Rlf-FLAG This paper N/A TT2 mESCs Zfp292-FLAG This paper N/A TT2 mESCs Zfp654-FLAG This paper N/A TT2 mESCs Rcor1-FLAG This paper N/A TT2 mESCs Rcor2-FLAG This paper N/A TT2 mESCs Rlf/Zfp292 DKO Rcor2-FLAG This paper N/A TT2 mESCs Rest-dTAG-FLAG This paper N/A Oligonucleotides See Table S2 for all oligonucleotides This paper N/A (Continued on next page) 16 Cell Reports 45, 117293, April 28, 2026 .. TT2 mESCs are kindly gifted from Dr. Tachibana in Osaka university. mESCs were cultured on dishes coated with 0.1% gelatin in DMEM (Nacalai tesque, 08458-16) supplemented with 10% Knockout Serum Replacement (Gibco, 10828028), 1% FBS (HyClone, SH30070.03), 1× sodium pyruvate (Wako, 190–14881), 1× non-essential amino acids (Wako, 139–15651), 0.1 mM 2-mercaptoethanol (Wako, 133–14571), 1× penicillin-streptomycin (Gibco, 15140122), and LIF (produced in-house) at 37◦C with 5% CO2. mESCs were passaged every 2–3 days using 0.05% trypsin-EDTA (Nacalai tesque, 32777-44).

    Purification:

    Article Title: RLF/ZFP292 stabilize CoREST-linked LSD1 engagement at bivalent promoters to safeguard pluripotency.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays In-Fusion HD Cloning Kit Takara 639650 DNA Ligation Kit ver. .. 2.1 Takara 6022 Lipofectamine 3000 Thermo Fisher Scientific L3000008 Leukocyte Alkaline Phosphatase Kit Sigma-Aldrich 86R ReverTra Ace qPCR RT Master Mix TOYOBO FSQ-201 TB Green Premix Ex Taq II Takara RR820A SYBR Green qPCR Master Mix Thermo Fisher Scientific A66732 QIAquick PCR Purification Kit Qiagen 28104 NEBNext Ultra II DNA Library Prep Kit for Illumina NEB E7645L NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) NEB E7600S NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 2) NEB E7780S NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) NEB E6440S Deposited data Raw and analyzed data (crosslinked IP-MS) This paper PXD075871 Raw and analyzed data (native IP-MS) This paper PXD075910 Raw and analyzed data (ChIP-seq) This paper GSE297949 Raw and analyzed data (RNA-seq) This paper GSE297950 REST ChIP-seq in mouse ESCs Arnold et al.37 GSE27148 ENCODE Blacklist for mm10 genome Amemiya et al.55 https://github.com/Boyle-Lab/Blacklist/ blob/master/lists/mm10-blacklist.v2.bed.gz ChromHMM_mESC_mm10 Pintacuda et al.36 https://github.com/guifengwei/ ChromHMM_mESC_mm10/blob/master/ mESC_E14_12_segments.bed.gz Original western blot images and fluorescence microscopy images This paper Mendeley data https://doi.org/ 10.17632/2j3895t9rh.1 Experimental models: Cell lines TT2 mESCs WT Gifted from Dr. Tachibana RRID: CVCL_C318 TT2 mESCs Rlf KO This paper N/A TT2 mESCs Zfp292 KO This paper N/A TT2 mESCs Rlf/Zfp292 DKO This paper N/A TT2 mESCs Rex1-EGFP This paper N/A TT2 mESCs Rlf/Zfp292 DKO Rex1-EGFP This paper N/A TT2 mESCs Zfp292 KO + Rlf-dTAG-2HA This paper N/A TT2 mESCs Zfp292/Zfp654 DKO + Rlf-dTAG-2HA This paper N/A TT2 mESCs Rlf-FLAG This paper N/A TT2 mESCs Zfp292-FLAG This paper N/A TT2 mESCs Zfp654-FLAG This paper N/A TT2 mESCs Rcor1-FLAG This paper N/A TT2 mESCs Rcor2-FLAG This paper N/A TT2 mESCs Rlf/Zfp292 DKO Rcor2-FLAG This paper N/A TT2 mESCs Rest-dTAG-FLAG This paper N/A Oligonucleotides See Table S2 for all oligonucleotides This paper N/A (Continued on next page) 16 Cell Reports 45, 117293, April 28, 2026 .. TT2 mESCs are kindly gifted from Dr. Tachibana in Osaka university. mESCs were cultured on dishes coated with 0.1% gelatin in DMEM (Nacalai tesque, 08458-16) supplemented with 10% Knockout Serum Replacement (Gibco, 10828028), 1% FBS (HyClone, SH30070.03), 1× sodium pyruvate (Wako, 190–14881), 1× non-essential amino acids (Wako, 139–15651), 0.1 mM 2-mercaptoethanol (Wako, 133–14571), 1× penicillin-streptomycin (Gibco, 15140122), and LIF (produced in-house) at 37◦C with 5% CO2. mESCs were passaged every 2–3 days using 0.05% trypsin-EDTA (Nacalai tesque, 32777-44).

    Chromatin Immunoprecipitation:

    Article Title: RLF/ZFP292 stabilize CoREST-linked LSD1 engagement at bivalent promoters to safeguard pluripotency.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays In-Fusion HD Cloning Kit Takara 639650 DNA Ligation Kit ver. .. 2.1 Takara 6022 Lipofectamine 3000 Thermo Fisher Scientific L3000008 Leukocyte Alkaline Phosphatase Kit Sigma-Aldrich 86R ReverTra Ace qPCR RT Master Mix TOYOBO FSQ-201 TB Green Premix Ex Taq II Takara RR820A SYBR Green qPCR Master Mix Thermo Fisher Scientific A66732 QIAquick PCR Purification Kit Qiagen 28104 NEBNext Ultra II DNA Library Prep Kit for Illumina NEB E7645L NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) NEB E7600S NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 2) NEB E7780S NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) NEB E6440S Deposited data Raw and analyzed data (crosslinked IP-MS) This paper PXD075871 Raw and analyzed data (native IP-MS) This paper PXD075910 Raw and analyzed data (ChIP-seq) This paper GSE297949 Raw and analyzed data (RNA-seq) This paper GSE297950 REST ChIP-seq in mouse ESCs Arnold et al.37 GSE27148 ENCODE Blacklist for mm10 genome Amemiya et al.55 https://github.com/Boyle-Lab/Blacklist/ blob/master/lists/mm10-blacklist.v2.bed.gz ChromHMM_mESC_mm10 Pintacuda et al.36 https://github.com/guifengwei/ ChromHMM_mESC_mm10/blob/master/ mESC_E14_12_segments.bed.gz Original western blot images and fluorescence microscopy images This paper Mendeley data https://doi.org/ 10.17632/2j3895t9rh.1 Experimental models: Cell lines TT2 mESCs WT Gifted from Dr. Tachibana RRID: CVCL_C318 TT2 mESCs Rlf KO This paper N/A TT2 mESCs Zfp292 KO This paper N/A TT2 mESCs Rlf/Zfp292 DKO This paper N/A TT2 mESCs Rex1-EGFP This paper N/A TT2 mESCs Rlf/Zfp292 DKO Rex1-EGFP This paper N/A TT2 mESCs Zfp292 KO + Rlf-dTAG-2HA This paper N/A TT2 mESCs Zfp292/Zfp654 DKO + Rlf-dTAG-2HA This paper N/A TT2 mESCs Rlf-FLAG This paper N/A TT2 mESCs Zfp292-FLAG This paper N/A TT2 mESCs Zfp654-FLAG This paper N/A TT2 mESCs Rcor1-FLAG This paper N/A TT2 mESCs Rcor2-FLAG This paper N/A TT2 mESCs Rlf/Zfp292 DKO Rcor2-FLAG This paper N/A TT2 mESCs Rest-dTAG-FLAG This paper N/A Oligonucleotides See Table S2 for all oligonucleotides This paper N/A (Continued on next page) 16 Cell Reports 45, 117293, April 28, 2026 .. TT2 mESCs are kindly gifted from Dr. Tachibana in Osaka university. mESCs were cultured on dishes coated with 0.1% gelatin in DMEM (Nacalai tesque, 08458-16) supplemented with 10% Knockout Serum Replacement (Gibco, 10828028), 1% FBS (HyClone, SH30070.03), 1× sodium pyruvate (Wako, 190–14881), 1× non-essential amino acids (Wako, 139–15651), 0.1 mM 2-mercaptoethanol (Wako, 133–14571), 1× penicillin-streptomycin (Gibco, 15140122), and LIF (produced in-house) at 37◦C with 5% CO2. mESCs were passaged every 2–3 days using 0.05% trypsin-EDTA (Nacalai tesque, 32777-44).

    RNA Sequencing:

    Article Title: RLF/ZFP292 stabilize CoREST-linked LSD1 engagement at bivalent promoters to safeguard pluripotency.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays In-Fusion HD Cloning Kit Takara 639650 DNA Ligation Kit ver. .. 2.1 Takara 6022 Lipofectamine 3000 Thermo Fisher Scientific L3000008 Leukocyte Alkaline Phosphatase Kit Sigma-Aldrich 86R ReverTra Ace qPCR RT Master Mix TOYOBO FSQ-201 TB Green Premix Ex Taq II Takara RR820A SYBR Green qPCR Master Mix Thermo Fisher Scientific A66732 QIAquick PCR Purification Kit Qiagen 28104 NEBNext Ultra II DNA Library Prep Kit for Illumina NEB E7645L NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) NEB E7600S NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 2) NEB E7780S NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) NEB E6440S Deposited data Raw and analyzed data (crosslinked IP-MS) This paper PXD075871 Raw and analyzed data (native IP-MS) This paper PXD075910 Raw and analyzed data (ChIP-seq) This paper GSE297949 Raw and analyzed data (RNA-seq) This paper GSE297950 REST ChIP-seq in mouse ESCs Arnold et al.37 GSE27148 ENCODE Blacklist for mm10 genome Amemiya et al.55 https://github.com/Boyle-Lab/Blacklist/ blob/master/lists/mm10-blacklist.v2.bed.gz ChromHMM_mESC_mm10 Pintacuda et al.36 https://github.com/guifengwei/ ChromHMM_mESC_mm10/blob/master/ mESC_E14_12_segments.bed.gz Original western blot images and fluorescence microscopy images This paper Mendeley data https://doi.org/ 10.17632/2j3895t9rh.1 Experimental models: Cell lines TT2 mESCs WT Gifted from Dr. Tachibana RRID: CVCL_C318 TT2 mESCs Rlf KO This paper N/A TT2 mESCs Zfp292 KO This paper N/A TT2 mESCs Rlf/Zfp292 DKO This paper N/A TT2 mESCs Rex1-EGFP This paper N/A TT2 mESCs Rlf/Zfp292 DKO Rex1-EGFP This paper N/A TT2 mESCs Zfp292 KO + Rlf-dTAG-2HA This paper N/A TT2 mESCs Zfp292/Zfp654 DKO + Rlf-dTAG-2HA This paper N/A TT2 mESCs Rlf-FLAG This paper N/A TT2 mESCs Zfp292-FLAG This paper N/A TT2 mESCs Zfp654-FLAG This paper N/A TT2 mESCs Rcor1-FLAG This paper N/A TT2 mESCs Rcor2-FLAG This paper N/A TT2 mESCs Rlf/Zfp292 DKO Rcor2-FLAG This paper N/A TT2 mESCs Rest-dTAG-FLAG This paper N/A Oligonucleotides See Table S2 for all oligonucleotides This paper N/A (Continued on next page) 16 Cell Reports 45, 117293, April 28, 2026 .. TT2 mESCs are kindly gifted from Dr. Tachibana in Osaka university. mESCs were cultured on dishes coated with 0.1% gelatin in DMEM (Nacalai tesque, 08458-16) supplemented with 10% Knockout Serum Replacement (Gibco, 10828028), 1% FBS (HyClone, SH30070.03), 1× sodium pyruvate (Wako, 190–14881), 1× non-essential amino acids (Wako, 139–15651), 0.1 mM 2-mercaptoethanol (Wako, 133–14571), 1× penicillin-streptomycin (Gibco, 15140122), and LIF (produced in-house) at 37◦C with 5% CO2. mESCs were passaged every 2–3 days using 0.05% trypsin-EDTA (Nacalai tesque, 32777-44).

    Western Blot:

    Article Title: RLF/ZFP292 stabilize CoREST-linked LSD1 engagement at bivalent promoters to safeguard pluripotency.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays In-Fusion HD Cloning Kit Takara 639650 DNA Ligation Kit ver. .. 2.1 Takara 6022 Lipofectamine 3000 Thermo Fisher Scientific L3000008 Leukocyte Alkaline Phosphatase Kit Sigma-Aldrich 86R ReverTra Ace qPCR RT Master Mix TOYOBO FSQ-201 TB Green Premix Ex Taq II Takara RR820A SYBR Green qPCR Master Mix Thermo Fisher Scientific A66732 QIAquick PCR Purification Kit Qiagen 28104 NEBNext Ultra II DNA Library Prep Kit for Illumina NEB E7645L NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) NEB E7600S NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 2) NEB E7780S NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) NEB E6440S Deposited data Raw and analyzed data (crosslinked IP-MS) This paper PXD075871 Raw and analyzed data (native IP-MS) This paper PXD075910 Raw and analyzed data (ChIP-seq) This paper GSE297949 Raw and analyzed data (RNA-seq) This paper GSE297950 REST ChIP-seq in mouse ESCs Arnold et al.37 GSE27148 ENCODE Blacklist for mm10 genome Amemiya et al.55 https://github.com/Boyle-Lab/Blacklist/ blob/master/lists/mm10-blacklist.v2.bed.gz ChromHMM_mESC_mm10 Pintacuda et al.36 https://github.com/guifengwei/ ChromHMM_mESC_mm10/blob/master/ mESC_E14_12_segments.bed.gz Original western blot images and fluorescence microscopy images This paper Mendeley data https://doi.org/ 10.17632/2j3895t9rh.1 Experimental models: Cell lines TT2 mESCs WT Gifted from Dr. Tachibana RRID: CVCL_C318 TT2 mESCs Rlf KO This paper N/A TT2 mESCs Zfp292 KO This paper N/A TT2 mESCs Rlf/Zfp292 DKO This paper N/A TT2 mESCs Rex1-EGFP This paper N/A TT2 mESCs Rlf/Zfp292 DKO Rex1-EGFP This paper N/A TT2 mESCs Zfp292 KO + Rlf-dTAG-2HA This paper N/A TT2 mESCs Zfp292/Zfp654 DKO + Rlf-dTAG-2HA This paper N/A TT2 mESCs Rlf-FLAG This paper N/A TT2 mESCs Zfp292-FLAG This paper N/A TT2 mESCs Zfp654-FLAG This paper N/A TT2 mESCs Rcor1-FLAG This paper N/A TT2 mESCs Rcor2-FLAG This paper N/A TT2 mESCs Rlf/Zfp292 DKO Rcor2-FLAG This paper N/A TT2 mESCs Rest-dTAG-FLAG This paper N/A Oligonucleotides See Table S2 for all oligonucleotides This paper N/A (Continued on next page) 16 Cell Reports 45, 117293, April 28, 2026 .. TT2 mESCs are kindly gifted from Dr. Tachibana in Osaka university. mESCs were cultured on dishes coated with 0.1% gelatin in DMEM (Nacalai tesque, 08458-16) supplemented with 10% Knockout Serum Replacement (Gibco, 10828028), 1% FBS (HyClone, SH30070.03), 1× sodium pyruvate (Wako, 190–14881), 1× non-essential amino acids (Wako, 139–15651), 0.1 mM 2-mercaptoethanol (Wako, 133–14571), 1× penicillin-streptomycin (Gibco, 15140122), and LIF (produced in-house) at 37◦C with 5% CO2. mESCs were passaged every 2–3 days using 0.05% trypsin-EDTA (Nacalai tesque, 32777-44).

    Fluorescence:

    Article Title: RLF/ZFP292 stabilize CoREST-linked LSD1 engagement at bivalent promoters to safeguard pluripotency.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays In-Fusion HD Cloning Kit Takara 639650 DNA Ligation Kit ver. .. 2.1 Takara 6022 Lipofectamine 3000 Thermo Fisher Scientific L3000008 Leukocyte Alkaline Phosphatase Kit Sigma-Aldrich 86R ReverTra Ace qPCR RT Master Mix TOYOBO FSQ-201 TB Green Premix Ex Taq II Takara RR820A SYBR Green qPCR Master Mix Thermo Fisher Scientific A66732 QIAquick PCR Purification Kit Qiagen 28104 NEBNext Ultra II DNA Library Prep Kit for Illumina NEB E7645L NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) NEB E7600S NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 2) NEB E7780S NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) NEB E6440S Deposited data Raw and analyzed data (crosslinked IP-MS) This paper PXD075871 Raw and analyzed data (native IP-MS) This paper PXD075910 Raw and analyzed data (ChIP-seq) This paper GSE297949 Raw and analyzed data (RNA-seq) This paper GSE297950 REST ChIP-seq in mouse ESCs Arnold et al.37 GSE27148 ENCODE Blacklist for mm10 genome Amemiya et al.55 https://github.com/Boyle-Lab/Blacklist/ blob/master/lists/mm10-blacklist.v2.bed.gz ChromHMM_mESC_mm10 Pintacuda et al.36 https://github.com/guifengwei/ ChromHMM_mESC_mm10/blob/master/ mESC_E14_12_segments.bed.gz Original western blot images and fluorescence microscopy images This paper Mendeley data https://doi.org/ 10.17632/2j3895t9rh.1 Experimental models: Cell lines TT2 mESCs WT Gifted from Dr. Tachibana RRID: CVCL_C318 TT2 mESCs Rlf KO This paper N/A TT2 mESCs Zfp292 KO This paper N/A TT2 mESCs Rlf/Zfp292 DKO This paper N/A TT2 mESCs Rex1-EGFP This paper N/A TT2 mESCs Rlf/Zfp292 DKO Rex1-EGFP This paper N/A TT2 mESCs Zfp292 KO + Rlf-dTAG-2HA This paper N/A TT2 mESCs Zfp292/Zfp654 DKO + Rlf-dTAG-2HA This paper N/A TT2 mESCs Rlf-FLAG This paper N/A TT2 mESCs Zfp292-FLAG This paper N/A TT2 mESCs Zfp654-FLAG This paper N/A TT2 mESCs Rcor1-FLAG This paper N/A TT2 mESCs Rcor2-FLAG This paper N/A TT2 mESCs Rlf/Zfp292 DKO Rcor2-FLAG This paper N/A TT2 mESCs Rest-dTAG-FLAG This paper N/A Oligonucleotides See Table S2 for all oligonucleotides This paper N/A (Continued on next page) 16 Cell Reports 45, 117293, April 28, 2026 .. TT2 mESCs are kindly gifted from Dr. Tachibana in Osaka university. mESCs were cultured on dishes coated with 0.1% gelatin in DMEM (Nacalai tesque, 08458-16) supplemented with 10% Knockout Serum Replacement (Gibco, 10828028), 1% FBS (HyClone, SH30070.03), 1× sodium pyruvate (Wako, 190–14881), 1× non-essential amino acids (Wako, 139–15651), 0.1 mM 2-mercaptoethanol (Wako, 133–14571), 1× penicillin-streptomycin (Gibco, 15140122), and LIF (produced in-house) at 37◦C with 5% CO2. mESCs were passaged every 2–3 days using 0.05% trypsin-EDTA (Nacalai tesque, 32777-44).

    Microscopy:

    Article Title: RLF/ZFP292 stabilize CoREST-linked LSD1 engagement at bivalent promoters to safeguard pluripotency.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays In-Fusion HD Cloning Kit Takara 639650 DNA Ligation Kit ver. .. 2.1 Takara 6022 Lipofectamine 3000 Thermo Fisher Scientific L3000008 Leukocyte Alkaline Phosphatase Kit Sigma-Aldrich 86R ReverTra Ace qPCR RT Master Mix TOYOBO FSQ-201 TB Green Premix Ex Taq II Takara RR820A SYBR Green qPCR Master Mix Thermo Fisher Scientific A66732 QIAquick PCR Purification Kit Qiagen 28104 NEBNext Ultra II DNA Library Prep Kit for Illumina NEB E7645L NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) NEB E7600S NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 2) NEB E7780S NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) NEB E6440S Deposited data Raw and analyzed data (crosslinked IP-MS) This paper PXD075871 Raw and analyzed data (native IP-MS) This paper PXD075910 Raw and analyzed data (ChIP-seq) This paper GSE297949 Raw and analyzed data (RNA-seq) This paper GSE297950 REST ChIP-seq in mouse ESCs Arnold et al.37 GSE27148 ENCODE Blacklist for mm10 genome Amemiya et al.55 https://github.com/Boyle-Lab/Blacklist/ blob/master/lists/mm10-blacklist.v2.bed.gz ChromHMM_mESC_mm10 Pintacuda et al.36 https://github.com/guifengwei/ ChromHMM_mESC_mm10/blob/master/ mESC_E14_12_segments.bed.gz Original western blot images and fluorescence microscopy images This paper Mendeley data https://doi.org/ 10.17632/2j3895t9rh.1 Experimental models: Cell lines TT2 mESCs WT Gifted from Dr. Tachibana RRID: CVCL_C318 TT2 mESCs Rlf KO This paper N/A TT2 mESCs Zfp292 KO This paper N/A TT2 mESCs Rlf/Zfp292 DKO This paper N/A TT2 mESCs Rex1-EGFP This paper N/A TT2 mESCs Rlf/Zfp292 DKO Rex1-EGFP This paper N/A TT2 mESCs Zfp292 KO + Rlf-dTAG-2HA This paper N/A TT2 mESCs Zfp292/Zfp654 DKO + Rlf-dTAG-2HA This paper N/A TT2 mESCs Rlf-FLAG This paper N/A TT2 mESCs Zfp292-FLAG This paper N/A TT2 mESCs Zfp654-FLAG This paper N/A TT2 mESCs Rcor1-FLAG This paper N/A TT2 mESCs Rcor2-FLAG This paper N/A TT2 mESCs Rlf/Zfp292 DKO Rcor2-FLAG This paper N/A TT2 mESCs Rest-dTAG-FLAG This paper N/A Oligonucleotides See Table S2 for all oligonucleotides This paper N/A (Continued on next page) 16 Cell Reports 45, 117293, April 28, 2026 .. TT2 mESCs are kindly gifted from Dr. Tachibana in Osaka university. mESCs were cultured on dishes coated with 0.1% gelatin in DMEM (Nacalai tesque, 08458-16) supplemented with 10% Knockout Serum Replacement (Gibco, 10828028), 1% FBS (HyClone, SH30070.03), 1× sodium pyruvate (Wako, 190–14881), 1× non-essential amino acids (Wako, 139–15651), 0.1 mM 2-mercaptoethanol (Wako, 133–14571), 1× penicillin-streptomycin (Gibco, 15140122), and LIF (produced in-house) at 37◦C with 5% CO2. mESCs were passaged every 2–3 days using 0.05% trypsin-EDTA (Nacalai tesque, 32777-44).



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